Sentence with the word cosmid
E. coli and 25 µg/ml for M. smegmatis; and hygromycin B, 100 µg/ml for oppA (Rv1280c) of M. tuberculosis was amplified from cosmid MTCY50 using the primer pair
The alphaT2ib open reading frame was integrated into an adenoviral cosmid vector for production of recombinant adenovirus (AdV) - alphaT2ib.
However, the linearized cosmid produced an incomplete assembly because the two DNA termini, though greatly over-represented in the clone library used for sequencing, were separated from neighboring sequences by gaps of ~1. 4 and 1.8 kb.
These gap sizes were reduced, but not eliminated, by shearing the linear cosmid into smaller fragments.
1987 (Burke et al. 1987) permitted much larger segments of DNA to be ordered and stored for sequencing than was possible with plasmid or cosmid libraries.
- Sentence for "cosmid"
- E. coli and 25 µg/ml for…
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- Hypernym for "cosmid"